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. 2018 Apr 27;12:997–1008. doi: 10.2147/DDDT.S151029

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© 2018 Ju et al. This work is published and licensed by Dove Medical Press Limited

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Effects of 4-amino-2-trifluoromethyl-phenyl retinate (ATPR) on the invasion and migration of MDA-MB-231 cells after silencing and overexpression of FABP5.

Notes: (A) Boyden chamber assay. MDA-MB-231 cells – with FABP5 silenced or overexpressed – were plated in the invasion chamber. After 20 h, cells remaining in the upper chamber were removed and cells that invaded through the Matrigel were fixed and stained using 0.5% crystal violet in methanol, ***P < 0.001 vs FL−/siRNA− group. (B) Wound healing assay. MDA-MB-231 cells – with FABP5 silenced or overexpressed – were subjected to a wound assay and monitored for 48 h. FL−: cells untreated with FABP5 transfection; FL+: cells treated with FABP5 transfection. (C) Immunofluorescence staining of FABP5 expression after treatment with ATPR (8 µmol/L) for 48 h (200×). One-way ANOVA, ***P < 0.001 vs the non-ATPR intervention group.