Fig. 6.

Inhibition ELISA of immunized interferons. A) Inhibition ELISA of anti-(rIFN-α2b, IFN-α2b,) immune (Δ, ◻) and preimmune (◼, ▲) sera with rIFNα-2b, IFN α-2b. B) Inhibition of immune anti-(rIFNα-2b, IFN α-2b) IgG binding to rIFN-α2b (Δ), IFN-α2b (◻). (◼, ▲) represent the inhibition of pre-immune anti-rIFN-α2b and IFN-α2bIgG binding to rIFN-α2b and IFN-α2b. The experiments were carried out by incubating the ELISA plate with 100 (l of respective antigens (2.5 μg/ml) as described in Material and methods