Figure 10.

Detection of PvLEA-18-related proteins in different plant species by western analysis. Protein extracts obtained from the seeds of the different species described below were separated by SDS-PAGE and transferred to nitrocellulose before incubation with the immunopurified anti-PvLEA-18 antiserum. PvLEA-18 cross-reacting proteins in dry seeds from: lane 1, cv Cacahuate of bean; lane 2, cv Flor de Mayo of bean; lane 3, cv Negro jamapa of bean; lane 4, tobacco; lane 5, maize; lane 6, Arabidopsis; lane 8, tomato; lane 9, soybean; and lane 10, pea. For S. lepidophylla, the protein extract was obtained from vegetative tissues of desiccated whole plants (lane 7). Numbers at both sides indicate the corresponding molecular masses in kilodaltons.