Figure 3.

Analysis of the accumulation of the Pvlea-18 transcript during germination. Bean seeds were germinated in the dark and seedlings were harvested at different times after imbibition (0, 2, 3, and 5 d postimbibition). Five micrograms of total RNA was purified from different seed or seedling organs, blotted onto nylon membranes, and hybridized as indicated. Hybridization against a 28S-rRNA probe was used as an RNA-loading control. a, Northern-blot analysis of RNA extracts obtained from the hypocotyl-root system. b, Northern-blot analysis of total RNA extracts obtained from cotyledons and plumules.