Figure 6.

Pvlea-18 transcript and protein accumulation patterns in different hypocotyl regions from seedlings grown under well-irrigated and water-stress conditions. E1 and E2, Hypocotyl-growing regions; M, nongrowing or mature zone; R, root. a, Northern-blot analysis of the Pvlea-18 transcript. Five-day-old bean seedlings grown in the dark were transferred to well-irrigated (C) or water-deficient (WD) vermiculite and harvested after 24 h of treatment. Five micrograms of total RNA was purified from the indicated seedling regions, blotted onto nylon membranes, and hybridized against the indicated probes. Hybridization against a 28S-rRNA probe was used as an RNA-loading control. b, PvLEA-18 protein accumulation pattern obtained by western analysis of total protein extracts purified from the same samples described in a. The molecular mass of PvLEA-18 is indicated on the right in kilodaltons. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes before incubation with the immunopurified anti-PvLEA-18 antiserum. c, Northern-blot analysis of the PvleaIV-25 transcript by blotting total RNA obtained from the samples described in a. Five micrograms of total RNA was blotted onto nylon membranes and hybridized against the indicated probes. Hybridization against an 28S-rRNA probe was used as an RNA-loading control.