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. 2018 Apr 30;13(4):e0194385. doi: 10.1371/journal.pone.0194385

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© 2018 Fischer et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Cut-off values were evaluated by testing of 239 negative control sera (NiV N based ELISA) and 154 sera (sHeV and sNiV G based ELISAs). The horizontal red broken line represents the cut-off value defined as mean OD value of all tested negative control sera plus three standard deviations. OD values were measured at 450 nm for the sHeV G and sNiV G as well as at 405 nm for the NiV N based ELISAs.