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. 2018 Apr 30;13(4):e0193922. doi: 10.1371/journal.pone.0193922

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© 2018 Aamir et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — A. Histochemical staining for observation of accumulated H₂O₂ in control and Fol toxins exposed leaf tissues (DAB staining) at 48 hrs post inoculation of Fol toxins. K, L and M. General view of control leaf samples. Q, R and S. microscopic observation of control leaf tissues. N and O. Fol toxins exposed leaf tissues (attached) showing the accumulated H₂O₂ along the midrib, leaf margins and tips. T and U. Microscopic observation of the treated tissues accumulating higher amount of H₂O₂ along the midrib and leaf tips. P and V. Higher accumulation of H2O2 in leaf tissues (de-attached) 5B. Biochemical assessment of H₂O₂ produced at different time interval. The H₂O₂ produced was higher at 24 hrs, become maximum at 48 hrs and decreases successively at increased time interval. The control tissues had more or less similar amount of H₂O₂ produced.