Fig 6. Histochemical analysis for the assessment of cell death.

The leaves (both attached and detached with plants) were treated with culture filtrate of (Fol). The microscopic observation of the Fol toxins exposed necrotic lesions developed that eventually leads into cell death and was observed using Evan Blue stain. A and B. General view of control leaf samples. C and D. General view of Fol toxins exposed leaf tissues (attached) with having intense blue coloration showed the dead tissues. E. General view of Fol toxins exposed and unattached leaf tissue. F and G. Microscopic observation of control leaf samples. H and I. The microscopic observation of the leaf tissues (attached) showing the binding of Evans dye with dead tissues. J De-attached leaves observed at higher resolution.