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. 2018 Apr 30;13(4):e0196626. doi: 10.1371/journal.pone.0196626

Fig 1. β-AR stimulation induces post-translational modifications of NF2 in ARVMs.

Fig 1

ARVMs were treated with ISO (10 μM) for 15 mins. Total cell lysates were analyzed by western blot using phospho-specific anti-NF2 antibodies. This antibody recognized ~70 kDa (phosphorylated NF2; A) and ~100 kDa (phosphorylated and sumoylated NF2; B) proteins. The lower panels exhibit the mean data normalized to total NF-2 (T-NF2) or GAPDH, *p<0.05 vs CTL; n = 3–7. (C) To confirm NF2 sumoylation, cell lysates were immunoprecipitated with anti-NF2 antibodies. Immunoprecipitates were analyzed by western blot using anti-SUMO-1 antibodies. Panel C exhibits quantitative increase in NF2 sumoylation in response to ISO. *p<0.05 vs CTL; n = 3.