Fig 7. Knockdown of NF2 using siRNA decreases β-AR-stimulated increase in NF2 and YAP phosphorylation.

H9C2 cells were transfected with NF2 siRNA for 48 h followed by treatment with ISO for 15 min. Cell lysates were analyzed by western blot using anti-NF2 (A), phospho-specific anti-NF2 (B) and phospho-specific anti-YAP (C) antibodies. The lower panels exhibit the mean data normalized to GAPDH, *P<0.05 vs.CTL; ^$p<0.05 vs. ISO; n = 3.