Fig 1. Identification of differentially decaying operons in E. coli.

(A-C), Differential decay in three representative E. coli operons, depicted by normalized RNA-seq coverage in steady state (black, t = 0) or at two time points (green and red) following rifampicin treatment. RNA-seq coverage was normalized by the number of uniquely mapped reads in each library. Bar graphs show average half-life calculations from three replicates with error bars representing standard deviation. (D), Ratio of steady-state mRNA abundance (blue) and mRNA half-lives (red) shown for a subset of regulated gene-pairs in which mRNA abundances and decay rates closely matched. Gene names are marked below the x-axis. Shown is the average ratio between the genes with error bars denoting standard deviation.