Fig 3. The csA mutant produces less viable spores than the wild-type strain.

Strains were inoculated into liquid sporulation media with the total cell count (spores and vegetative bacteria) (coloured bars) and spore count alone (grey bars) determined every 24 hours. At each time point all strains show similar total cell counts, however the csA mutant produces less spores than the wild-type strain. The results represent the average of four independent experiments and error bars represent standard error of the mean. Asterisks indicate statistical difference in comparison to WT at *, p ≤ 0.05; **, p ≤ 0.01. Wild-type, WT; csA mutant, csA-M; csB mutant, csB-M; csA mutant complemented, csA-C.