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. 1999 Jun;120(2):371–382. doi: 10.1104/pp.120.2.371

Figure 2.

Figure 2

POX isoforms identified in IEF- and native-PAGE. IEF-PAGE (A) and native-PAGE (B) gels were loaded with filtrate medium of 7-d tobacco BY-2 cell-suspension culture (lanes M) and purified fractions corresponding to POX acidic isoforms A1 and A2. The amounts of proteins loaded onto the IEF-PAGE gels were 1 μg (lane M) and 250 ng (lanes A1 and A2), and the amounts loaded onto the native-PAGE gels were 1 μg (lane M), 250 ng (lane A1), and 150 ng (lane A2). Peroxidase activity was revealed using chloro-1-naphthol as a substrate.