Table II.
Protein | Anionic Exchanger Mono-Q
|
Cationic Exchanger Mono-S
|
Hydrophobic-Interaction Phenyl Superose
|
Gel-Filtration Superdex 75
|
Purification Factor | ||||
---|---|---|---|---|---|---|---|---|---|
Elution peak | Specific activity | Elution peak | Specific activity | Elution peak | Specific activity | Molecular mass | Specific activity | ||
mM NaCl | units μg−1 protein | mM NaCl | units μg−1 protein | m (NH4)2SO4 | units μg−1 protein | kD | units μg−1 protein | ||
A1 | 60 | 59.1 | 0.57 | 165.3 | 45 | 180.6 | 258 | ||
A2 | 38 | 45.2 | |||||||
B1 | 16 | 1.7 | 0.80 | 5.6 | 33.5 | 7.1 | 10 | ||
B2 | 46 | 23.4 | 0.44 | 93.5 | 33 | 107.8 | 154 | ||
B3 | 68 | 74.1 | 0.59 | 122.0 | 33 | 137.2 | 196 | ||
P1 | 130 | 112.1 | 1.36 | 133.2 | 42 | 146.4 | 209 | ||
P2 | 143 | 168.0 | 1.09 | 198.8 | 40 | 213.7 | 305 |
NaCl concentrations corresponding to peaks of elution of each isoPOX are indicated, together with specific activities using o-dianisidine as a substrate, as described in Methods.