Table II.

Purification of tobacco POXs from BY-2 cell-suspension culture medium: fast-protein liquid chromatography steps

Protein	Anionic Exchanger Mono-Q		Cationic Exchanger Mono-S		Hydrophobic-Interaction Phenyl Superose		Gel-Filtration Superdex 75		Purification Factor
	Elution peak	Specific activity	Elution peak	Specific activity	Elution peak	Specific activity	Molecular mass	Specific activity
	mM NaCl	units μg−1 protein	mM NaCl	units μg−1 protein	m (NH4)2SO4	units μg−1 protein	kD	units μg−1 protein
A1	60	59.1			0.57	165.3	45	180.6	258
A2	38	45.2
B1			16	1.7	0.80	5.6	33.5	7.1	10
B2			46	23.4	0.44	93.5	33	107.8	154
B3			68	74.1	0.59	122.0	33	137.2	196
P1			130	112.1	1.36	133.2	42	146.4	209
P2			143	168.0	1.09	198.8	40	213.7	305

NaCl concentrations corresponding to peaks of elution of each isoPOX are indicated, together with specific activities using o-dianisidine as a substrate, as described in Methods.