Figure 4.

Confirmation of macrophages as predominant HSP90α-expressing CD11b⁺-myeloid cells during PDAC development. (A) Duplex in situ hybridization assays showing that F4/80⁺ cells rather than Ly6G⁺ cells were the HSP90α-expressing CD11b⁺ myeloid cells in the pancreatic tissues of LSL-KrasG12D/Pdx1-Cre mice at 3 months of age. The HSP90α⁺ cells with simultaneous CD11b⁺, F4/80⁺, or Ly6G⁺ were not detected from the pancreatic tissues of 1-month-old LSL-KrasG12D/Pdx1-Cre mice and 1- or 3-month-old LSL-KrasG12D mice. (B) Quantification of the cells with HSP90α⁺, CD11b⁺, F4/80⁺, and Ly6G⁺, respectively, from the pancreatic tissue sections described in (A). (C) Quantification of the % of HSP90α⁺ cells from CD11b⁺, F4/80⁺, and Ly6G⁺ cells, respectively, from the pancreatic tissue sections of LSL-KrasG12D/Pdx1-Cre mice at 3 months of age. (D) Quantification of the % of CD11b⁺, F4/80⁺, and Ly6G⁺ cells, respectively, from HSP90α⁺ cells from the pancreatic tissue sections of LSL-KrasG12D/Pdx1-Cre mice at 3 months of age.