Figure 1.

Influenza a virus infection mediates oncolysis of raf-transformed lung cancer tissue in vivo. Raf-BxB mice were infected with 500 particles of influenza A virus PR8 and at different days post infection lungs were analyzed for: (A and B), expression of human Raf-BxB and viral NP by immunohistochemistry; (C), number of tumor foci per lung; (D), relative size of tumor foci; (E), amount of human Raf-BxB mRNA transcripts by TaqMan qRT-PCR; (F), relative expression of oncogenic Raf-BxB and viral MI RNA. Mean values of uninfected control mice were arbitrarily taken as unity. Mean values ± SEM of two experiments with ≥5 animals per group in each experiment are presented. Always serial paraffin lung sections were used for stainings of Raf-BxB and NP. Bars = 2000 μm (A); 100 µm (B)