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. 2018 Feb 12;7(5):e1423171. doi: 10.1080/2162402X.2017.1423171

Figure 5.

Figure 5.

Anti-IAV immune responses in the lungs of WT and tumor-bearing Raf-BxB mice. C57Bl/6 WT and Raf-BxB mice were infected with 500 particles of IAV PR8 and at indicated times, the BALF were analyzed for presence of immune cells per se as well as for distinct populations of lymphocytes and alveolar macrophages. The absolute number of total immune cells (A) and alveolar macrophages (CD11b−/Ly6C−/Ly6G−/CD11chi/F4/80+) per mouse are shown. Percentages of MHCII+(C), iNOS+ (D) and IL4Rα+ (E) alveolar macrophages are depicted. Representative dot-blot images underlining the gating strategy are shown in Figure S1. (F, G) mRNA expression levels of Csf2 and Ccl2 in total lung lysates were analyzed by TaqMan qRT-PCR. mRNA expression levels in uninfected WT mice were arbitrarily taken as unity. Mean values ± SEM of two experiments with ≥5 animals per group in each experiment are shown.