Figure 4.

Antitumor efficacy and overall survival in a clinically relevant set up after the combination of virotherapy with checkpoint blockade. (A) 12–16 animals per group received subcutaneous B16.OVA tumors that were grown for 10 days. Then mice received i.t. 1 × 10⁸ viral particles of non-replicating adenoviruses coding for mIL2 and mTNFa or PBS. The same day, depending on the group they belonged, they received 0.1 mg of anti-PD-1. Checkpoint blockade treatment was repeated every 3 days for a total of 13–15 times. At day 4, 7 untreated animals engrafted with the same tumors were sacrificed to study the status of the tumor at “baseline” time point. 6–7 random animals from each group were euthanized at day 11 (grey line) and organs were collected for further analysis, remaining animals were maintained for survival studies. (B) Overall survival and statistical significances. (C) Individual tumor growth lines for the different conditions tested and statistical significance of the differences between groups at day 39.