Figure 7.

In vivo, CMT167 cells but not LLC cells express MHC class II genes and cofactors necessary for MHC class II processing and presentation. CMT167 tumors and LLC tumors were implanted in GFP-expressing transgenic mice. (A) After several weeks, cancer cells were recovered by sorting for GFP-negative cells using flow cytometry. Total RNA was isolated, analyzed by RNA-seq, and compared with total RNA collected from cancer cells grown in vitro. CMT167 and LLC cancer cells were analyzed for surface expression of MHC II by flow cytometry. The histograms show overlays of MHC II expressed by CMT167 cells (shaded) and LLC cells (white) in vitro (B) and in vivo (C). Data are representative of six independent in vitro experiments and three independent in vivo experiments (n = 2 mice per group).