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. 2018 Feb 1;7(5):e1423170. doi: 10.1080/2162402X.2017.1423170

Figure 3.

Figure 3.

PD-L1 expression is not regulated by conventional EMT transcriptional factors in A549. (A) Expression of several transcription factors of EMT as: ZEB-1, SNAIL-1, SLUG, GLI1, GLI2, TWIST in protein levels was measured by Western Blotting after 5 days of treatment of A549 cells with TNF-α and TGFβ. This experiment was realized 3 times. (B) qRT-PCR analysis was realized to test the expression of different transcription factors of EMT mRNA levels after treatment with cytokines. GAPDH was used as a housekeeping gene. (C) The expression of VIMENTIN and E-CADHERIN were measured using Western-blotting in A549 cells after transfection with SNAIL-1 or control vector. (D) expression of EPCAM and PD-L1 was measured by FACS analysis during treatment with TNF-α and TGFβ on A549 cancer cells transfected with SNAIL-1 or control vector. Results from Fig. 3A and 3 C and 3D are representative examples from at least 3 independent experiments.