Figure 5.

PD-L1 expression during EMT is dependent from NF-kB in signaling in A549 cells. (A) Activity of NF-kB was measured in A549 cells treated or not with TNF-α and TGFβ1. (B) The expression of NF-kB subunits was measured by Western Blotting after treatment with NF-kB inhibitor. (C) The expression of PD-L1 was measured using cytometry following NF-kB inhibition with JSH-23. (D) Expression of EPCAM and PD-L1 was measured using cytometry in A549 treated or not with TNF-α and TGFβ1 and transfected or not with si IKKε. These experiments were repeated tree times with similar results.