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. 2018 Jan 29;7(5):e1423184. doi: 10.1080/2162402X.2017.1423184

Figure 4.

Figure 4.

Accumulation of CD161-expressing CD3+ T cells within NSCLC tumor stroma and at the vicinity of TLS. (A-B) IF staining of a frozen section from NSCLC tumor using anti-human CD161 clone DX12 (B) or isotype control (A) associated with DAPI. (C-E, H) Double IF stainings of serial frozen sections from the same NSCLC tumor using anti-human CD161 clone DX12 associated with (C) anti-human CD20, (D-E, H) anti-human CD3, and (H) DAPI. (E) represents a higher magnification (x400) of area (white rectangle) in (D). (F) Double IF staining of a serial frozen section from the same NSCLC tumor using anti-human LLT1 clone 2F1 associated with anti-human CD20. (G) ImageJ-processed overlay of LLT1 IF staining from (F) and CD161/CD20 double IF staining from (C), at high magnification. (I-J) Triple IF stainings of frozen sections from two NSCLC tumors using anti-human CD161 clone DX12 associated with anti-human CD20 and anti-human CD3. (A-G, I-J) focus on TLS. (H) focus on tumor stroma and tumor nests. (A-D, F, H-J) magnification x200. (E, G) magnification x400.