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. Author manuscript; available in PMC: 2018 Apr 30.
Published in final edited form as: Mol Pharm. 2017 Sep 26;14(11):3815–3823. doi: 10.1021/acs.molpharmaceut.7b00559

Figure 2.

Figure 2

TMV-PEG8/28-STK characterization. (A–C) SDS–PAGE and Western Blot (WB) analysis of TMV-Lys particles before and after conjugation of STK. Free STK was used as reference. Bands corresponding to TMV capsid protein (TMVcp, MW = 17 kDa) and STK (MW = 47 kDa) are indicated with arrows. Successful TMV-STK conjugation is indicated by presence of multiple protein bands corresponding to TMVcp-PEG8/28-STK (multiple bands of apparent MW > 64 kDa; theoretical molecular weight of 1:1 SA:TMVcp monomer = 64 kDa) as shown by WB immune recognition. The low molecular weight bands (<14 kDa; panels A and B) correspond to TMV coat protein fragments, possibly resulting from degradation during sample preparation. (D, E) TEM images of TMV-PEG28-STK and TMV-PEG8-STK particles, respectively.