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. Author manuscript; available in PMC: 2018 Apr 30.
Published in final edited form as: Adv Exp Med Biol. 2017;966:103–148. doi: 10.1007/5584_2017_93

Figure 5.

Figure 5

Amino acid specificity spectra of Class I LeuRS and Class II HisRS2 Urzymes.

The mean net free energy for specificity of Class I and II Urzymes for homologous vs. heterologous substrates, i.e., ΔG(kcat/KM)ref – ΔG(kcat/KM)amino acid (i), where ref is the cognate amino acid, is approximately 1 kcal/mole for both Class I and II Urzymes (center). Class I amino acids are colored blue; Class II amino acids are colored green. Bold colors denote substrates from the homologous Class; pastel colors denote heterologous substrates. (From Carter, CW Jr., (2015) MDPI Life 5: 294–320).