Figure 3.

Toxic shRNAs derived from seven TS genes induce death that is biochemically similar to DISE.

Quantification of ROS production by dichlorofluorescin (DCFH) fluorescence (A), caspase-2 activity (B) and quantification of cell death with PI staining (C) in HeyA8 cells 8 days after infection with shScr, shL3, and shRNAs derived from eight of the TS genes. ID numbers are shown in Table 1. p-values were calculated using students t-test. *p < 0.01, **p < 0.001, ***p < 0.0001. For genes in black, both shRNAs were functionally active whereas genes shown in grey, only one of the two shRNAs had a significant effect. Color code for the two shRNAs per gene is the same as in Fig. 1.