Figure 2.

NEAT1 knockdown in hibited cisplatin resistance of NPC cells. The expression level of NEAT1 in S18 (A) and 5–8F (B) cells was obviously downregulated by transfecting with sh-NEAT1. Cell viability was determined by MTT assay in S18 (C) and 5–8F (D) cells after transfection with sh-NEAT1 or sh-NC, following exposing to 10 μg/ml of cisplatin. The representative fluorescent images of EdU assay in S18 (E) and 5–8F (F) cells were shown. EdU assay showed that downregulation of NEAT1 inhibited cell proliferation ability in S18 (G) and 5–8F (H) cells in the presence of 10 μg/ml of cisplatin. *P < 0.05, **P < 0.01, and ***P < 0.001.