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. 2018 Apr 3;17(4):515–526. doi: 10.1080/15384101.2018.1431594

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Figure 4. — IGF-1 can maintain RPCs in an undifferentiated state in the proliferation condition. (A) The mRNA expression levels of Nestin, Vimentin, Pax-6 and Chx10 in the RPCs cultured with IGF-1 plus EGF or EGF were evaluated by qPCR analysis, and the results showed that there were no obvious differences between the two groups. (B) Western blot analysis of the expression levels of Nestin, Vimentin and Pax6 in the RPCs cultured with IGF-1 plus EGF or EGF. (C-E) Immunocytochemistry analysis of the ratios of Nestin- and Vimentin-positive cells in the RPCs cultured with IGF-1 plus EGF or EGF. The results showed that there were no remarkable differences between the two groups. Scale bar: 100 μm.