Figure 2.
Relative movement ratio analysis of the Dα1 GAL4-UAS rescue system. Following the protocol of Denecke, et al., 2015, 3rd instar larvae were bathed in (A) 5% sucrose or (B) a 5% sucrose:25 ppm imidacloprid solution, and their movement recorded over time.17 The differences in movement relative to their starting activity is presented in the graph as a relative movement ratio. No difference is observed between the three genotypes in 5% sucrose solution across the duration of the experiment. At 25 ppm imidacloprid all three genotypes show a rapid and sustained reduction in movement. Both the background control and rescue larvae have a much greater reduction in relative movement when compared the reporter-only knockout.