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. 2018 Jan 29;12(2):109–117. doi: 10.4161/19336918.2014.990781

Figure 3.

Figure 3.

G-1 up regulated the expression of MMP2 and MMP-9 in RCC cells via GPER. (A) ACHN cells were treated with 1 μM G-1 for the indicated times, and then the protein levels of MMP-2 and MMP-9 were measured by Western blotting; (B) OS-RC-2 cells were treated with 1 μM G-1 for the indicated times, and then the protein levels of MMP-2 and MMP-9 were measured by Western blotting; ACHN cells were transfected with GPER specific si-RNA (si-GPER) or negative control si-RNA (si-NC) for 24 h before treated with or without 1 μM G-1 for 48 h, and then the proteins levels of GPER, MMP-2 and MMP-9 were measured by Western blotting (C), and the number of migrated cells were detected by use of transwell chamber (D). Data represent at least three independent experiments. **P < 0.01 compared with control.