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. 2018 Jan 29;12(2):109–117. doi: 10.4161/19336918.2014.990781

Figure 6.

Figure 6.

PI3K/AKT/MMP-9 mediated the G-1 induced migration of RCC cells. (A) ACHN cells were pretreated with PD98059 (10 μM) or LY294002 (10 μM) for 90 min respectively followed by stimulation with 1 μM G-1 for 48 h. The expression of MMP-9 was examined by Western blot analysis. (B) OS-RC-2 cells were pretreated with PD98059 (10 μM) or LY294002 (10 μM) for 90 min respectively followed by stimulation with 1 μM G-1 for 48 h. The expression of MMP-9 was examined by Western blot analysis. (C) ACHN cells were pretreated with inhibitors of MMP-9 (Cat-444278, 10 nM) and/or LY294002 (10 μM) for 90 min respectively followed by stimulation with 1 μM G-1 for 48 h. The number of migrated cells were compared with the control. Data are presented as means ± SD of three independent experiments. *P < 0.05 compared with control; **P < 0.01 compared with control.