Table 1.

Known binding partners of Drosophila Eya and So.

Eya Binding Partner	Symbol	Yeast two-hybrid	in vitro binding	Co-IP	References
Combgap	Cg	Positive	Positive	Negative	[10]
Dachshund	Dac	Positive	Positive	Positive	[2-4]
Eyeless	Ey	Untested	Untested	Positive	[4]
Eyes absent	Eya	Untested	Untested	Positive	[6,8]
Groucho	Gro	Untested	Positive	Negative	[5,8]
I-κB kinase β	IKKβ	Untested	Untested	Positive	[11]
Optix	Optix	Negative	Untested	Positive	[1,5]
Relish	Rel	Untested	Untested	Positive	[11]
Sine oculis	So	Positive	Positive	Positive	[1,2,4-8]
So Binding Partner	Symbol	Yeast two-hybrid	in vitro binding	Co-IP	References
Eyeless	Ey	Untested	Positive	Untested	[9]
Eyes absent	Eya	Positive	Positive	Positive	[1,2,4-8]
Groucho	Gro	Positive	Untested	Positive	[5,8]
Optix binding protein	Opbp	Positive	Positive	Untested	[5]
Sine oculis binding protein	Sobp	Positive	Positive	Untested	[5]
TBP-associated factor 1	Taf1	Positive	Positive	Untested	[5]

We considered yeast two-hybrid, direct in vitro binding assays, and co-immunoprecipitation experiments to be evidence of complex formation. (A) Proteins that bind Eya. (B) Proteins that bind So. ¹Anderson, A. M., Weasner, B. M., Weasner, B. P. and Kumar, J. P. (2012). Dual transcriptional activities of SIX proteins define their roles in normal and ectopic eye development. Development 139, 991–1000. ²Bui, Q. T., Zimmerman, J. E., Liu, H. and Bonini, N. M. (2000). Molecular analysis of Drosophila eyes absent mutants reveals features of the conserved Eya domain. Genetics 155, 709–20. ³Chen, R., Amoui, M., Zhang, Z. and Mardon, G. (1997). Dachshund and eyes absent proteins form a complex and function synergistically to induce ectopic eye development in Drosophila. Cell 91, 893–903. ⁴Jin, M. and Mardon, G. (2016). Distinct Biochemical Activities of Eyes absent During Drosophila Eye Development. Sci. Rep. 6, 23228. ⁵Kenyon, K. L., Li, D. J., Clouser, C., Tran, S. and Pignoni, F. (2005). Fly SIX-type homeodomain proteins Sine oculis and Optix partner with different cofactors during eye development. Dev. Dyn. 234, 497–504. ⁶Mutsuddi, M., Chaffee, B., Cassidy, J., Silver, S. J., Tootle, T. L. and Rebay, I. (2005). Using Drosophila to decipher how mutations associated with human branchio-oto-renal syndrome and optical defects compromise the protein tyrosine phosphatase and transcriptional functions of eyes absent. Genetics 170, 687–95. ⁷Pignoni, F., Hu, B., Zavitz, K. H., Xiao, J., Garrity, P. a and Zipursky, S. L. (1997). The eye-specification proteins So and Eya form a complex and regulate multiple steps in Drosophila eye development. Cell 91, 881–91. ⁸Silver, S. J., Davies, E. L., Doyon, L. and Rebay, I. (2003). Functional Dissection of Eyes absent Reveals New Modes of Regulation within the Retinal Determination Gene Network. Mol. Cell. Biol. 23, 5989–5999. ⁹Zhang, T., Ranade, S., Cai, C. Q., Clouser, C. and Pignoni, F. (2006). Direct control of neurogenesis by selector factors in the fly eye: regulation of atonal by Ey and So. Development 133, 4881–9. ¹⁰Davis, T. L. and Rebay, I. (2017). Antagonistic regulation of the second mitotic wave by Eyes absent-Sine oculis and Combgap coordinates proliferation and specification in the Drosophila retina. Development. ¹¹Liu, X., Sano, T., Guan, Y., Nagata, S., Hoffmann, J. A. and Fukuyama, H. (2012). Drosophila EYA Regulates the Immune Response against DNA through an Evolutionarily Conserved Threonine Phosphatase Motif. PLoS One 7, e42725.