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. 2018 Feb 8;17(4):479–485. doi: 10.1080/15384101.2017.1415678

Figure 2.

Figure 2.

А. Western-blot analysis of CSML-100 surface proteins. В. Western-blot analysis of CSML-100 surface proteins after their interaction with PGRPs. C. Western-blot analysis of recombinant proteins: Hsp70, S100A4, Hsp70 and S100A4 after their interaction with a lymphocyte. А and В. CSML-100 surface proteins were biotinylated; results are visualized using Streptavidin-Horseradish Peroxidase. C. Recombinant Hsp70 and S100A4 are biotinylated; formed complexes are extracted on AbPGRPs-sepharose. D. S100A4 concentration on the CSML-100 cells before and after their stimulation with PGRPs. The S100A4 content in the samples was measured by ELISA. The stimulation was performed by adding 20 ng/ml PGRPs into the CSML-100 incubation medium for 24 hours. Error bars show SEM based on five biological replicates.