Figure 3.

A. S100A4 impact on target cells survival. The activated lymphocytes (20:1) were added to CSML-100 and melanoma M3 cells that were pre-cultivated with S100A4 antibodies and incubated for 3 hours at 37°C. The cells survival was measured with an MTT test in five independent experiments. Error bars are ±SEM. B. The lymphocytes were extracted from the peripheral blood of healthy donors and incubated for 6 d with 1000 U/ml interleukin-2 or with interleukin-2 and 10 μ/ml paclitaxel. Cultivated and not cultivated with paclitaxel lymphocytes were added to К562 cells in the presence of 1 mM recS100A4, and in the presence or absence of 1mM Ca²⁺. *p < 0.05. Error bars show SEM based on five biological replicates. С. Paclitaxel impact on CSML-100 cells survival in the presence of cultivated and not cultivated with paclitaxel lymphocytes. The part of CSML-100 cells was pre-cultivated with paclitaxel (10 μ/ml), and then cultivated and not cultivated with paclitaxel lymphocytes were added. Error bars show SEM based on five biological replicates, *p < 0.05.