Figure 3. Presynaptic homeostatic potentiation can be induced and expressed exclusively at synapses innervating muscle 6.

(A) Schematic and electrophysiological traces of recordings from muscles 7 and 6 of wild type and M6 > GluRIIA^RNAi NMJs. Note that mEPSPs are reduced only on muscle 6 of M6 > GluRIIA^RNAi, while EPSP amplitudes are similar across all muscles. Thus, the expression of PHP is restricted to synapses innervating muscle 6 of M6 > GluRIIA^RNAi and does not impact neurotransmission at neighboring synapses within the same motor neuron. (B) Quantification of mEPSP amplitude and quantal content values of M6 > GluRIIA^RNAi normalized to wild-type values. (C–E) Quantification of mEPSP amplitude (C), EPSP amplitude (D), and quantal content (E) in the indicated muscles and genotypes. Asterisks indicate statistical significance using a Student’s t test: (****) p<0.0001, (ns) not significant. Error bars indicate ± SEM. Detailed statistical information for represented data (mean values, SEM, (n, p) is shown in Supplementary file 1.