Figure 4. PHP can be induced and expressed acutely on muscle 7 following chronic GluRIIA knock down on M6.

(A) Schematic and representative traces illustrating the acute application of PhTx on wild type and M6 >GluRIIA^RNAi NMJs. The acute expression of PHP was observed on previously non-potentiated muscle 7 synapses in M6 >GluRIIA^RNAi NMJs. mEPSP amplitudes are diminished at wild type and M6 >GluRIIA^RNAi NMJs on both muscles following PhTx application, while EPSP amplitudes are maintained at baseline levels due to a homeostatic increase in presynaptic neurotransmitter release. (B) Quantification of mEPSP and quantal content values normalized to baseline values (-PhTx) at muscles 6 and 7 NMJs of M6 >GluRIIA^RNAi larvae. (C–E) Quantification of mEPSP amplitude (C), EPSP amplitude (D), and quantal content (E) values in the indicated genotypes. Asterisks indicate statistical significance using a Student’s t test and one-way analysis of variance (ANOVA), followed by Tukey’s multiple-comparison test: (**) p<0.01, (****) p<0.001, (ns) not significant. Error bars indicate ± SEM. Detailed statistical information for represented data (mean values, SEM, (n, p) is shown in Supplementary file 1.