Figure 7. Compartmentalized changes in CaMKII activity at postsynaptic densities of M6 > GluRIIA^RNAi NMJs.

(A) Representative images of muscle 6/7 NMJs from wild type and M6 > GluRIIA^RNAi immunostained with antibodies against with the active (phosphorylated) form of CaMKII (pCaMKII) and the presynaptic neuronal membrane marker HRP. Note that pCaMKII intensity is specifically reduced at Ib postsynaptic densities on muscle 6 of M6 >GluRIIA^RNAi (dashed areas). (B,C) Images (B) and quantification (C) of pCaMKII fluorescence intensity at Ib boutons on muscles 7 and 6 normalized to wild-type values. A reduction in pCaMKII intensity is observed on muscle 6 of M6 > GluRIIA^RNAi, while no significant change is observed at synapses innervating the adjacent muscle 7. (D,E) Images (D) and quantification (E) of pCaMKII intensity at Is boutons shows no significant difference between wild type and M6 > GluRIIA^RNAi at NMJs of either muscle. Asterisks indicate statistical significance using a Student’s t test: (***) p<0.001, (ns) not significant. Error bars indicate ± SEM. Detailed statistical information for represented data (mean values, SEM, (n, p) is shown in Supplementary file 1.