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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Insect Biochem Mol Biol. 2018 Mar 9;95:10–16. doi: 10.1016/j.ibmb.2018.02.005

Fig. 9. Effect of sugar feeding on insemination rates.

Fig. 9

A) Virgin males were fed a 0% or a 20% sucrose solution for 6 days. Individual males were left undisturbed for 45 min with three virgin females to allow mating to occur. Fifteen males were evaluated for each treatment. B) Virgin males were fed a 0% or a 20% sucrose solution for 6 days. After that, starved males were given 20% sugar for 24 h, and insemination rates were determined at 7 days for both groups. Fifteen males were evaluated for each treatment. Positive insemination rates were determined by dissecting female spermathecae and evaluating the presence of sperm. Bars represent the means ± SEM of the number of females (0 to 3) inseminated by individual males (n = 15). Asterisk denotes significant difference (unpaired t-test; * P ≤ 0.05). NS denotes no significant difference (unpaired t-test).