Cohorts of 5-9 GF or SPF Il10-/- mice were gavaged with a single dose of 109 CFU C. jejuni/mouse and were euthanized 12 or 21 days post-infection. SPF Il10-/-mice were treated with an antibiotics (Abx) cocktail in their drinking water for 7 days before infection. (A) H&E staining showing representative intestinal histology of C. jejuni-induced colitis in SPF (left), SPF+ Abx (middle) and GF (GF, right) Il10-/- mice. (B) Quantification of histological intestinal damage score. (C) Colonic Il1β, Cxcl2, and Il17a mRNA qPCR fold change relative to GF and normalized to Gapdh. **, P<0.01. Scale bar is 200 μm. Results are representative of 3 independent experiments.