Table 2.
Strains used in this study.
| Strain Name | Relevant Genotypea | Source or Reference |
|---|---|---|
| Legionella pneumophila | ||
| JR32 | Philadelphia-1; Smr; r- m+ | 89 |
| KS79 (WT) | JR32 ∆comR | 96 |
| ∆letS (GAH338) | KS79 letS::aptII, Knr | 65 |
| ∆letS + pletS (SPF39) | ∆letS + pMMB207c Ptac-letS; Cmr | 65 |
| ∆rsmYZ (SPF41) | KS79 rsmY::aptII, rsmZ::aacC1; Knr, Gmr | This study |
| ∆rsmYZ + prsmY (SPF291) | ∆rsmYZ + pXDC39-prsmY; Knr, Gmr, Cmr | This study |
| ∆rpoS | JR32 rpoS::Tn903dGent; Gmr | 96 |
| ∆rpoS + prpoS | ∆rpoS + pMMB207c Ptac-rpoS; Cmr | 14 |
| ∆relA∆spoT (ppGpp°) | KS79 ∆relA::aacC1 spot::aptII; Gmr, Kmr | 14 |
| Plasmid Name | ||
| pBBR1MCS-5 | pBBR1MCS Gmr | 97 |
| pSF6 | DH5α, pGEMT-easy-rrnb | 98 |
| pMMB207c | RSF1010 derivative, IncQ, lacIq Cmr Ptac oriT ∆mobA | 99 |
| pXDC39 | pMMB207c ∆Ptac, ∆lacI, Cmr | Xavier Charpentier |
| prsmY | pXDC39-rsmY, Cmr | This study |
aSmr, streptomycin resistance; Cmr, chloramphenical resistance; Gmr, gentamicin resistance; Kmr, kanamycin resistance.