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. 2018 Apr 30;8:6780. doi: 10.1038/s41598-018-24671-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Wet AFM data obtained on PAAm sample formulation 3 showing a histogram of the spread of moduli values for 3 repeats of 100 indentations on a 5 × 5 μm sampling area and (b) box and whisker plot showing the overall spread across the range of PAAm samples tested. All AFM experiments were conducted with an indentation depth of between 500 and 1000 nm and each curve was done over 1 s, 900 repeats per formulation were conducted with three samples per formulation.