Fig. 1.

Identification of RNA binding proteins (RBPs) in primary human keratinocyte progenitors. a Schematic of the mRNA interactome-capture approach in primary human keratinocyte cultures. Three independent biological replicates were performed with pooled primary cultures of different donors. Cells were UV-cross-linked or nonirradiated and the bound to poly(A)-tagged mRNAs polypeptides were enriched and eluted from the oligo(dT) beads and further identified by LS-MS/MS. b Levels of 18s rRNA, β-actin, and hypoxanthine phosphoribosyltransferase 1 (HPRT) mRNAs in eluates from oligo(dT) captured samples were evaluated by qRT-PCR. Exogenous poly(A)-tailed luciferase mRNA was “spiked” into the lysates and used as an internal control. Error bars represent mean SD, n = 3. c Eluted proteins were visualized by SDS-PAGE and silver staining. d Western blotting on the eluted samples for levels of PTBP and histone H3. e RBPs expression profile in human keratinocytes during differentiation (³⁶, data accessible at NCBI GEO database, accession GSE58161)