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. 2018 Apr 30;8:6756. doi: 10.1038/s41598-018-25233-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree of Stx2 subtypes by the neighbor-joining method. The neighbor-joining tree was inferred from comparison of combined (A and B) holotoxin amino acid sequences of all Stx2 subtypes. Numbers on the tree indicate bootstrap values calculated for 1000 subsets for branch points >50%. Bar, 0.05 substitutions per site. Stx2 subtypes are indicated by different colors. An extended version of this tree is available as Fig. S2.