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. 2018 Apr 30;8:6756. doi: 10.1038/s41598-018-25233-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of Stx2h production in STEC299. (A) mRNA expression by qRT-PCR. The relative levels of expression under non-induction and induction conditions were relative to the expression level in Xuzhou21 before induction by mitomycin C which was arbitrary set at 1.0. The relative value was averaged from three independent experiments. Error bars represent the standard errors. (B) Vero toxicity assay. The cytotoxicity was detected after 24 h incubation exposure to the induced supernatants overnight. Xuzhou21 was used as positive control; MG1655 was used as negative control. (C) Western blot assays with an anti-Stx2 rabbit polyclonal antibody on whole cells and supernatants from STEC299 induced with mitomycin C with a final concentration of 0.5 μg/ml. Xuzhou21 was used as positive control.