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. 2018 Apr 30;8:6756. doi: 10.1038/s41598-018-25233-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The phylogenetic relationship of the strain STEC299 with the other 32 reference strains. (A) ClonalFrame tree of the stains inferred from the concatenated ribosomal protein gene sequences that are single-copy and shared (n = 53) by the 33 strains. Three independent and converged runs were merged and a 95% consensus tree was presented in the final graph. (B) Neighbor-net phylogeny generated from wgMLST allele profiles of 2,321 loci that shared by all the strains. The uncertainty and incompatibilities in the dataset were shown as networks. (C) Gubbins tree generated with the concatenated sequences of all the shared loci found in wgMLST analysis. STEC strains are highlighted in red on the three different trees.