Fig. 3.

Phylogenetic diversity of GSO 16S rRNA genes recovered from sulfidic and non-sulfidic stations from the Peruvian upwelling region. The phylogenetic tree was calculated using the neighbor joining and RAxML methods, including various filters, an unrooted consensus tree is shown. The typeface in blue, black, and green represent sequences recovered from other studies. Typeface in red represents sequences recovered from sulfidic stations U1 and U1a, and from offshore stations L1, L2, and 378 (Supplementary Table 1). The coverage and specificity of the newly designed FISH GSO131 probe is indicated by the red brackets; for overall probe coverage details see Supplementary Table 3. The broad coverage GSO477 probe is indicated by the gray bracket