Table 1.
Real-time PCR (RT-PCR) results of M. ulcerans DNA detection in aulacode feces (FAG), water (EAU) and water containing plant debris (VEG) by using detection of KR-B gene and insertion sequences IS2404 and IS2606.
| Samples | KR-B | IS2404 | IS2606 |
|---|---|---|---|
| KON-FAG 1 | +29.65 | ||
| KON-FAG 2 | |||
| KON-FAG 3 | |||
| KON-FAG 4 | +33.04 | +31.58 | |
| KON-FAG 5 | |||
| KON-FAG 6 | |||
| KON-FAG 7 | |||
| KON-FAG 8 | +36.30 | ||
| KON-FAG 9 | |||
| KON-FAG 10 | |||
| KON-FAG 11 | |||
| KON-EAU1 | +33.11 | +30.97 | +29.85 |
| KON-EAU 2 | +32.14 | +32.00 | +30.77 |
| KON-EAU 3 | |||
| KON-EAU 4 | +31.56 | +29.94 | |
| KON-EAU 5 | +31.36 | +29.64 | |
| KON-EAU 6 | +32.74 | +30.64 | |
| KON-EAU 7 | +30.67 | +29.18 | |
| KON-EAU 8 | +30.15 | +27.99 | |
| KON-EAU 9 | +30.15 | +30.08 | |
| KON-EAU 10 | +32.75 | +32.55 | +31.75 |
| KON-EAU 11 | +32.22 | +31.58 | +30.51 |
| KON-EAU 12 | +32.37 | +32.12 | +29.85 |
| KON-VEG 1 | +30.97 | ||
| KON-VEG 2 | +32.85 | ||
| KON-VEG 3 | |||
| KON-VEG 4 | +34.70 | +30.09 | |
| KON-VEG 5 | |||
| KON-VEG 6 | +32.34 | +31.64 | |
| KON-VEG 7 | |||
| KON-VEG 8 | +32.23 | +29.07 |
Ct values are indicated for specimens detected as positive.