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. 2018 Apr 30;8:6707. doi: 10.1038/s41598-018-25273-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of eIF4E-dependent in vitro translation by 4E-binding peptides. 1 pmol eIF4E (green) or 1 pmol eIF4E/p20 (red) was added to in vitro translation reactions containing 5 ng capped GRE1-Luc mRNA. Additionally, 1 pmol of the following purified GST-fusion proteins were added: GST-Eap1_106–273, GST-eIF4G_441–492, GST-eIF4G_160–492 and GST-eIF4G_160–492 LL457,458/AA. 1 pmol GST alone had no stimulatory effect on translation. Data presented are the mean luminescence activity in relative light units (RLU ± s.e.m.; n = 3).