Figure 5.

Effect of the N-terminal 18aa p20 peptide on translation and eIF4E binding of capped RNA. (a) Increasing amounts of the 18aa N-terminal p20 peptide (up to 5 pmol) were added to in vitro translational assays in the presence of 1 pmol eIF4E (green) or 1 pmol eIF4E/p20 (red). Translational assays were performed as described for Fig. 4. (b) MST: 50 nM RED fluorescence dye-labeled His6x-eIF4E in the absence (green) or presence of equimolar amounts of the 18aa N-terminal p20 peptide (yellow) with serially diluted m⁷GpppG-capped 64 nt long RNA (40 nt of SSA1 5′UTR), starting at 1 µM. Error bars indicate s.d. (n = 3).