Figure 4.

SF attenuated MPP⁺-mediated collapse of mitochondrial membrane permeability in SH-SY5Y cells. Cells were exposed to 1 mM MPP⁺ for 24 h in the absence or presence of 0.01 mg/mL SF for 24 h, stained with tetramethylrhodamine methyl ester perchlorate (TMRM), then visualized by fluorescence microscopy and the intensity of fluorescence was calculated. MPP⁺ collapsed mitochondrial membrane permeability, which was prevented by SF. Scale bar = 50 μm. Values are the means ± SD (three slides per group). ^a,bMeans with different letters are significantly different (p < 0.05) by Duncan’s multiple-range test.