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. 2018 Apr 24;9:218. doi: 10.3389/fneur.2018.00218

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Copyright © 2018 Huang, Liu, Peng, Dai, Xie, Chen, Long, Pei, Su and Yao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Circadian rhythm dysfunction (CRD) aggravated motor neuron (MN) loss and activated glial cells in SOD1G93A mice. (A) MNs [choline acetyltransferase (ChAT)-positive cells, red] and astrocytes [glial fibrillary acidic protein (GFAP)-positive cells, green] expression in four groups for three stages. (B) MNs (ChAT-positive cells, red) and microcytes (Iba1-positive cells, green) expression in four groups for three stages. Scale bars represent 100 µm. (C) Statistical evaluation of surviving MNs radio by counting the number of ChAT-positive cells. **p < 0.01; * p < 0.05 (one-way ANOVA, n = 4–6 animals per group). (D,E) GFAP and Iba1 expression was quantified at three stages by western blotting (one-way ANOVA, n = 2–3 animals per group). (F) Expression of GFAP and Iba1 in the spinal cord. **p < 0.01; *p < 0.05. Error bars represent SEM.